Olympus Fluoview FV-1000 
Inverted Laser Scanning microscope
Single photon point scanning Olympus FV1000 confocal microscope with three fluorescence and one transmitted light detectors 
PicoQuant Fluorescence Lifetime Microscopy (FLIM) upgrade with two SPAD detectors

 

special features confocal imaging: multicolor parallel and sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman averaging, summarizing, scan zoom and rotation
highly efficient scan mode with fast switching between imaging and activation for bleaching, photoactivation, FRAP
Time resolved fluorescence: Fluorescence Lifetime Microscopy (FLIM), Fluorescence Correlation Spectroscopy (FCS) 
microscope based on Olympus IX-81 inverted fully motorized fluorescence microscope, transmitted light with DIC or phase contrast (100x)
objectives 10x/0.4 UPlanApo, DIC
20x/0.7 UPlanApo, DIC
40x/1.35 UPlanSApo, DIC
60x/1.35 Oil UPlanSApo, DIC
100x/1.35 Oil UPlanSApo, Phase contrast
60x/1.2 Water UPlanSApo
illumination Halogen
HBO
405nm diode laser
458, 488, 515 ArgonLaser
561 nm diode laser
detection point scanner,  one pinhole, 3 confocal PMTs:
two independent spectral detection channels, each one configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection
one filter based detection channels, T-PMT, dichromatic mirrors for excitation/emission splitting

reflectors for widefield observation & TIRFM

Filter Cube 2. U-MWU2: BP330-385, BA420, DM400
Filter Cube 3. U-MCFPET: BP426-446, BA460-500, DM455
Filter Cube 4. U-MNB2: BP470-490, BA520IF, DM500
Filter Cube 5. U-MWG2: BP510-550, BA590, DM570
Filter Cube 6. DIC
dichroic filters for LSM 80/20
405/488/559/635
405-458/515/559/635
458/515
405/458/515
 

PicoQuant Fluorescence Lifetime Microscopy (FLIM) upgrade
illumination
picosecond-pulsed lasers
405nm diode laser
440nm diode laser
470nm diode laser
635nm diode laser
 
detection two higly sensitive (SPAD) detectors
resolvable time resolution: < 0.5 ns 
   
incubation incubation chamber for temperature (RT-37C), CO2 and humidity
temperature controlled holder for 35mm dishes and slights (waterbath controlled)
   
more Olympus information on FV1000
with free download of FV-1000 viewer software, can be used to view, arrange and convert Olympus image files