Differential Interference Contrast (DIC) for transmitted light.
DIC for transmitted light.
| Nikon TE-2000 |
| Inverted epifluorescence microscope for single molecule dection |
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| Manual research grade
fluorescence microscope with HBO and Diode-Laser widefield illumination,
detection with two higly sensitive Back-illuminated EMCCD cameras . Differential Interference Contrast (DIC) for transmitted light. |
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| special features | single molecule sensitivity, automated multicolor imaging, time series
with high time resolution DIC for transmitted light. |
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| microscope | Nikon Eclipse TE2000 inverted manual fluorescence microscope, transmitted light with DIC |
| objectives | 10x/0.30 PlanFluor (DIC) 40x/0.75 PlanFluor (DIC) 60x/1.40 Oil PlanApo VC (DIC) 60x/1.20 Water PlanApo VC (DIC) 100x/1.49 Oil Apo TIRF |
| illumination | Halogen HBO Diode Lasers 405, 440, 488, 563 and 635nm |
| detection | Two Andor Technology Back-illuminated EMCCD cameras iXon EM+ 128x128 pixels, up to 512 frames / s iXon EM+ 512x512 pixels, up to 35 frames / s |
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reflectors |
Filter Cube 1. laser imaging 488 and 635nm Filter Cube 2. laser imaging 488 and 561nm Filter Cube 3. HBO lamp imaging UV: BP320/40, BA390/40, DM355DCLP (DHE, Pyrene) only with special lamp and 60x/1.20 Water PlanApo VC objective Filter Cube 4. HBO lamp imaging "GFP": BP470/40, BP525/50, DM495 LP (GFP, FITC, NBD) Filter Cube 5. HBO lamp imaging "Rho": BP560/40, BP630/75, DM585 LP (Rhodamine, Texas Red, RFP) Filter Cube 6. HBO lamp imaging "CY5": BP620/60, BP700/75, DM660 LP (CY5) |
| software | Andor IQ (more information) and MetaMorph 7.8 |
| incubation | n.a. |
| more |
Andor information on
iXonEM+ cameras Nikon information on the Eclipse TE2000 microscope |