The Advanced Light Microscopy Facility (ALMF) is available to all workgroups of the Institute of Biology of the Humboldt-University Berlin. Providing sufficient free capacity ALFM also provides access to its equipment for other groups of the Humboldt-University Berlin and for collaborating users not associated with the Humboldt-University of Berlin.

We offer various methods of light microscopy, for instance epifluorescence microscopy, confocal laser scanning microscopy (CLSM) and spinning disk confocal laser microscopy (SDM). The instruments are equipped for the following advanced techniques:

- multiphoton confocal microscopy for deep tissue imaging
- time resolved methods: Fluorescence Lifetime Imaging (FLIM), Fluorescence Correlation Spectroscopy (FCS / FCCS)
- Anisotropy imaging
- Total Internal Reflection Microscopy (TIRFM)
- photo activation and fluorescence recovery after photo bleaching (FRAP)

Most of the instruments are equipped for life cell imaging with temperature, CO2 and humidity control.

We can also provide access to a Becton Dickinson FACS Aria II flow cytometer with cell high-speed sorting option (more information).

 For more details see "Equipment" and "Techniques".

Microscopes and additional equipment have been purchased by the Institute of Biology, Humboldt-University Berlin with financial support of the DFG, the BMBF, the IRI Life Sciences and other research supporting institutions.
Basic financing of the ALMF for repairs, maintenance and upgrades is provided by the Institute of Biology of the Humboldt-University Berlin. Project-specific equipment, supplies and extended usage within specific projects should be supported by these programs.

Currently, there are no usage fees for users of the Humboldt-University Berlin and for collaborating groups within academic scientific networks. External non-academic users have to be charged at a competitive rate, in case of interest please contact the ALMF supervisor Dr. Thomas Korte ( 



Invalidenstraße 42
Mittelbau Hof
10115 Berlin, Germany

Tel.: 030-2093-8830,
Fax: 030-2093-8585



aktualisiert: 18.04.2018  Thomas Korte