The Advanced Light Microscopy Facility (ALMF) is available to all workgroups of the Institute of Biology of the Humboldt-University Berlin. Providing sufficient free capacity ALFM also provides access to its equipment for other groups of the Humboldt-University Berlin and for collaborating users not associated with the Humboldt-University of Berlin.
We offer various methods of light
microscopy, for instance epifluorescence microscopy, confocal laser scanning
microscopy (CLSM) and spinning disk confocal laser microscopy (SDM). The
instruments are equipped for the following advanced techniques:
- multiphoton confocal microscopy for deep tissue imaging
- time resolved methods: Fluorescence Lifetime Imaging (FLIM), Fluorescence
Correlation Spectroscopy (FCS / FCCS)
- Anisotropy imaging
- Total Internal Reflection Microscopy (TIRFM)
- photo activation and fluorescence recovery after photo bleaching (FRAP)
Most of the instruments are equipped for life cell imaging with temperature, CO2 and humidity control.
We can also provide access to a Becton Dickinson FACS Aria II flow cytometer with cell high-speed sorting option (more information).
For more details see "Equipment"
and "Techniques".
Microscopes and
additional equipment have been purchased by the Institute of Biology,
Humboldt-University Berlin with financial support of the DFG, the BMBF, the IRI
Life Sciences and other research supporting institutions.
Basic financing of the ALMF for repairs, maintenance and upgrades is provided by
the Institute of Biology of the Humboldt-University Berlin. Project-specific
equipment, supplies and extended usage within specific projects should be
supported by these programs.
Currently, there are no usage fees for users of the Humboldt-University Berlin
and for collaborating groups within academic scientific networks.
External non-academic users have to be charged at a competitive rate, in case of
interest please contact the ALMF supervisor Dr. Thomas Korte (biologie-mikroskopie@hu-berlin.de).
Invalidenstraße 42
Mittelbau Hof
10115 Berlin, Germany
Tel.: 030-2093-8830,
Fax: 030-2093-8585
email: biologie-mikroskopie@hu-berlin.de
